| Anti-alpha Tubulin Mouse mAb | |
| Catalog number : | AT0007 |
| Size: | 100 µL |
| The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins. | |
| Tested applications | |
| WB : 1/1000-1/10000. Predicted molecular weight: 50 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
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| Properties | |
| Immunogen | |
| Recombinant full length protein, corresponding to amino acids 1-452 of Rat alpha Tubulin | |
| Clonality | |
| 4F3 | |
| Isotype | |
| Mouse IgG1 | |
| Form | |
| Liquid, 100 μg at 1mg/ml | |
| Storage instruction | |
| Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C | |
| Applications | |
| WB Image | |
| Western blot analysis of 40µg of cell lysates at 1:1000 dilution using Alpha Tubulin antibody Predicted band size: 50KDa, Observed band size: 50KDa Lane 1: A431 cells Lane 2: BT-474 cells Lane 3: NIH/T3 cells Lane 4: PC12 cells Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098) Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747) |
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